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Fig1: Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: Hela
Fig2: ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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