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細(xì)胞介紹
| 名稱 | SNU-387 (人肝癌細(xì)胞) (STR鑒定正確) |
| 別稱 | SNU387; NCI-SNU-387 |
| 種屬 | 人類 |
| 年齡（性別） | 女性，41歲 |
| 組織來源 | 肝癌 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 背景描述 | SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum. |
| 生物安全等級 | 2 |
| 生長培養(yǎng)基 | RPMI-1640＋10% FBS＋1% P/S |
| 推薦傳代比例 | 1:3-1:6 |
| 推薦換液頻率 | 2~3次/周 |
| 倍增時間 | ~61小時 (PubMed=7543080) |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮 |
| 培養(yǎng)條件 | 氣相：空氣，95%；CO2，5%溫度：37℃ |
| 保藏機構(gòu) | ATCC; CRL-2237 |
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