Fig1: Western blot analysis of TCF4 on different cell lysates using anti-TCF4 antibody at 1/1,000 dilution.

Fig2: ICC staining TCF4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining TCF4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TCF4 antibody. Counter stained with hematoxylin.

Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TCF4 antibody. Counter stained with hematoxylin.

Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCF4 antibody. Counter stained with hematoxylin.

Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TCF4 antibody. Counter stained with hematoxylin.

Fig8: Flow cytometric analysis of HepG2 cells with TCF4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.