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Fig1: Western blot analysis of Phosphotyrosine on A431 cells lysates treated (
Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.
Line 1) and untreated with Sodium metavanadate using anti- Phosphotyrosine antibody at 1/100 dilution.
Fig2: ICC staining Phosphotyrosine in A431 cells (green) nontreated (Left) or treated with Sodium metavanadate (Right). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of A431 cells with Phosphotyrosine antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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