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Fig1: Western blot analysis of Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) on different lysates using anti-Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: SH-SY-5Y
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: SH-SY-5Y
Fig2: ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of NIH/3T3 cells with Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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