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Fig1: Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: A431 Lane 3: Mouse heart
Fig2: ICC staining PDHA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PDHA1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining PDHA1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Fig10: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PDHA1 antibody. Counter stained with hematoxylin.
Fig11: Flow cytometric analysis of Hela cells with PDHA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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