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Fig1: Western blot analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7
Fig2: ICC staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Argonaute 2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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