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Fig1: Western blot analysis of FUS/TLS on K562 cells lysates using anti-FUS/TLS antibody at 1/1,000 dilution.
Fig2: ICC staining FUS/TLS in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining FUS/TLS in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining FUS/TLS in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FUS/TLS antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with FUS/TLS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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