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Fig1: Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Hela Lane 3: K562 Lane 4: Jurkat Lane 5: A431
Fig2: ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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