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Fig1: Western blot analysis of HNF-4-alpha on human kidney lysates using anti-HNF-4-alpha antibody at 1/1,000 dilution.
Fig2: ICC staining HNF-4-alpha in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of HepG2 cells with HNF-4-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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