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Fig1: Western blot analysis of Vimentin on different lysates using anti-Vimentin antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: A549
Lane 1: Hela
Lane 2: A549
Fig2: ICC staining Vimentin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Vimentin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Vimentin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: ICC staining Vimentin in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Vimentin antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of Hela cells with Vimentin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second antibody
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