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Fig1: Western blot analysis of MAP1LC3A on different lysates using anti-MAP1LC3A antibody at 1/1,000 dilution. Positive control:
Lane 1: SHG-44
Lane 2: Mouse brain
Lane 3: Mouse liver
Lane 4: Mouse skeletal muscle
Lane 1: SHG-44
Lane 2: Mouse brain
Lane 3: Mouse liver
Lane 4: Mouse skeletal muscle
Fig2: ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining MAP1LC3A in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining MAP1LC3A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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